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Fine-scale temporal dynamics of herpes virus and vibrios in seawater during a polymicrobial infection in the Pacific oyster Crassostrea gigas ArchiMer
Petton, Bruno; De Lorgeril, Julien; Mitta, Guillaume; Daigle, Gaétan; Pernet, Fabrice; Alunno Bruscia, Marianne.
The Pacific oyster Crassostrea gigas is currently being impacted by a polymicrobial disease that involves early viral infection by ostreid herpesvirus-1 (OsHV-1) followed by a secondary bacterial infection leading to death. A widely used method of inducing infection consists of placing specific pathogen-free oysters (‘recipients’) in cohabitation in the laboratory with diseased oysters that were naturally infected in the field (‘donors’). With this method, we evaluated the temporal dynamics of pathogen release in seawater and the cohabitation time necessary for disease transmission and expression. We showed that OsHV-1 and Vibrio spp. in the seawater peaked concomitantly during the first 48 h and decreased thereafter. We found that 1.5 h of cohabitation...
Tipo: Text Palavras-chave: Aquaculture; Bivalve; Epidemiology; Health; Polymicrobial disease; OSHV-1; Pacific oyster mortality syndrome; POMS.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00507/61870/65940.pdf
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Frequency of viral etiology in symptomatic adult upper respiratory tract infections BJID
Silva,Raquel Cirlene da; Mendes,Gabriella da Silva; Rojas,Miguel Angel; Amorim,Ariane Ribeiro; Couceiro,José Nelson; Lupi,Omar; Elabras,José; Pires,Gisele; Valle,Solange; Santos,Norma.
Aims: To determine the frequency of viral pathogens causing upper respiratory tract infections in non-hospitalized, symptomatic adults in the city of Rio de Janeiro.Methods:Respiratory samples (nasal/throat swabs) were collected between August 2010 and November 2012 and real time PCR was used to detect different viral pathogens.Results:Viruses were detected in 32.1% (43/134) of samples from 101 patients. Specifically, 9% (12/134) were positive for HBoV, 8.2% (11/134) were positive for HAdV, 5.2% (7/134) were positive for HRV, and 1.5% (2/134) were positive for FLUBV or HMPV, as single infections. HRSV-A, HPIV-3, and HCoV-HKU1 were detected in one (0.75%) sample each. Co-infections were detected in 4.8% (6/134) of the samples. Peaks of viral infections were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Respiratory infection; Viral infection; Viral diagnosis; Epidemiology.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000100030
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Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil Trop. Plant Pathol.
FAJARDO,THOR V. M.; NISHIJIMA,MARTA; BUSO,JOSÉ A.; TORRES,ANTÔNIO C.; ÁVILA,ANTÔNIO C.; RESENDE,RENATO O..
Garlic viruses often occur in complex infections in nature. In this study, a garlic virus complex, collected in fields in Brazil, was purified. RT-PCR was performed using specific primers designed from the consensus regions of the coat protein genes of Onion yellow dwarf virus, a garlic strain (OYDV-G) and Leek yellow stripe virus (LYSV). cDNA of Garlic common latent virus (GCLV) was synthesized using oligo-dT and random primers. By these procedures individual garlic virus genomes were isolated and sequenced. The nucleotide sequence analysis associated with serological data reveals the presence of two Potyvirus OYDV-G and LYSV, and GCLV, a Carlavirus, simultaneously infecting garlic plants. Deduced amino acid sequences of the Brazilian isolates were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: OYDV-G; LYSV; GCLV; Detection; Coat protein; PCR; Probes; Epidemiology.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000300007
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Genetic Similarities of Escherichia Coli Isolated from Different Substrates of the Broiler Production Chain Rev. Bras. Ciênc. Avic.
Teles,PFS; Boiago,MM; Frigo,A; Rampazzo,L; Araújo,DN; Kich,JD; Rebelatto,R; Furian,TQ; Stefani,LM.
ABSTRACT Brazil is the largest exporter of chicken meat and poultry farming is one of the most important productive segments, despite major losses due to the bacterium Escherichia coli, which is also a zoonotic microorganism. The objetive of this study was to isolate E. coli and to evaluate its transmissibility potential from the field to chicken meat using the Pulsed Field Gel Electrophoresis (PFGE) technique. Environmental samples (poultry litter, soil and water) were collected from broiler farms located in the South of Brazil where the majority of the Brazilian poultry production occurs. In addition, chicken meat (gizzard, heart, drumette and tulip) samples were collected from local supermarkets. As results, 47.36% of the samples were positives for E....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Environment; Epidemiology; Microbiology; Public health; Pulsed field gel electrophoresis.
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000100308
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Genotypic Diversity of the Wheat leaf blotch pathogen ( Septoria tritici ) in Buenos Aires Province Boletín de la Sociedad
Cordo,Cristina Alicia; Linde,Celeste C.; Zhan,Jashiu; Mcdonald,Bruce.
The population structure and genotypic diversity of Septoria tritici Rob ex Desm. from two crop field populations in Buenos Aires Province were studied with DNA restriction fragment length polymorphism. Hierarchical samples were taken from different cultivars. A total of 120 single spore isolates was subject to molecular analysis to compare the genetic structure of Los Hornos and Balcarce populations separated by 500km. Eight RFLP loci and 1DNA fingerprinting were used. Among the total of the isolates, 24 RFLP alleles were detected from the first population and 22 from the second. Gene and genotype diversity were high in both populations. Different haplotypes for each region were identified. Identical haplotypes were clustered in the same location in the...
Tipo: Info:eu-repo/semantics/other Palavras-chave: DNA hybridization; Septoria tritici; Mycosphaerella graminicola; RFLP; Epidemiology; Genetic diversity; Genotypic diversity.
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722006000200010
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Geostical analysis of spatial dynamics of Citrus Variegated Chlorossis Trop. Plant Pathol.
ROBERTO,SÉRGIO R.; FARIAS,PAULO R. S.; BERGAMIN FILHO,ARMANDO.
The spatial dynamics of Citrus Variegated Chlorosis (CVC) was studied in a five-year old commercial orchard of 'Valencia' sweet orange (Citrus sp.) trees, located in the northern region of the state of São Paulo, Brazil. One thousand trees were assessed in 25 rows of 40 trees, planted at 8 x 5 m spacing. Disease incidence data were taken beginning in March 1994 and ending in January 1996, at intervals of four to five months. Disease aggregation was observed through the dispersion index analysis (Ib), which was calculated by dividing the area into quadrants. CVC spatial dynamics was examined using semivariogram analysis, which revealed that the disease was aggregated in the field forming foci of 10 to 14 m. For each well-fitted model, a kriging map was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CVC; Xylella fastidiosa; Epidemiology.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000600007
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Glanders in donkeys (Equus asinus) in the state of Pernambuco, Brazil: a case report BJM
Mota,Rinaldo Aparecido; Oliveira,Andréa Alice da Fonseca; Pinheiro Junior,José Wilton; Silva,Leonildo Bento Galiza da; Brito,Marilene de Farias; Rabelo,Silvana Suely Assis.
The clinical, anatomical and histopatological findings of glanders diagnosis in donkeys in the state of Pernambuco-Brazil are reported. The animals were euthanized and necropsied, and evaluated for lesions in respiratory and lymphatic systems, confirming the disease by isolation of Burkholderia mallei and Strauss test.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Burkholderia mallei; Epidemiology; Microbiology; Histopathology; Strauss test.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100021
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Gray mold caused by Botrytis cinerea limits grape production in Chile Ciencia e Investigación Agraria
Latorre,Bernardo A; Elfar,Karina; Ferrada,Enrique E.
Gray mold (GM) caused by Botrytis cinerea is a major disease of grapes (Vitis vinifera) that substantially reduces the yield and quality of grape production in temperate and humid regions of the world. B. cinerea is a necrotrophic fungus that attacks the non-lignified aerial organs of grapes; in particular, berries are highly susceptible during ripening. The polycyclic nature and exponential progress exhibited by GM at the beginning of the its epidemic, as well as the abundant inoculum production, the high dissemination efficiency, the wide host range and the high genetic variability of B. cinerea, explain the difficulties encountered in attempting to control GM. At present, integrated disease management, including cultural and chemical control, is the...
Tipo: Journal article Palavras-chave: Botryotinia fuckeliana; Bunch rot; Epidemiology; Fungicides; Necrotroph; Vitis vinifera.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202015000300001
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Guinea pigs experimentally infected with vaccinia virus replicate and shed, but do not transmit the virus Ciência Rural
Cargnelutti,Juliana Felipetto; Wendlant,Adriéli; Weiblen,Rudi; Flores,Eduardo Furtado.
The origin of vaccinia viruses (VACV) associated with vesicular disease in cattle and humans in Southeast Brazil remains uncertain, yet the role of wild species in virus transmission has been suggested. This study investigated the susceptibility and transmission potential by guinea pigs (Cavia porcellus) - phylogenetically close to an abundant Brazilian rodent (Cavia aperea) - to two VACV strains (P1V and P2V) isolated from an outbreak of cutaneous disease in horses in Southern Brazil. Eight guinea pigs inoculated intranasally with P1V and P2V (10(6) TCID50.ml-1) did not develop clinical signs, but six animals shed virus in nasal secretions (day 1 to 9 post-inoculation - pi), developed viremia (between days 1 and 10 pi) and seroconverted to VACV. In spite...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Cavia porcellus; Cavia aperea; Orthopoxvirus; Transmission; Epidemiology.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000600017
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HBV epidemiology and genetic diversity in an area of high prevalence of hepatitis B in southern Brazil BJID
Paoli,Juliana de; Wortmann,André Castagna; Klein,Mirelli Gabardo; Pereira,Vagner Reinaldo Zingalli Bueno; Cirolini,Adriana Maria; Godoy,Bibiane Armiliato de; Fagundes,Nelson Jurandi Rosa; Wolf,Jonas Michel; Lunge,Vagner Ricardo; Simon,Daniel.
ABSTRACT Background Hepatitis B virus (HBV) infection is a major public health problem in Brazil. HBV endemicity is usually moderate to low according to geographic regions, and high prevalence of this virus has been reported in people of some specific Brazilian counties, including those with a strong influence of Italian colonization in southern Brazil. Analysis of HBV diversity and identification of the main risk factors to HBV infection are necessary to understand hepatitis B epidemiology in these high prevalence regions in southern Brazil. Objective To investigate epidemiological characteristics and HBV genotypes and subgenotypes circulating in a specific city with high HBV prevalence. Methods A cross-sectional study was performed with 102 HBV...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HBV infection; Epidemiology; HBV genotypes; Risk factor; Transmission; Cross-sectional studies.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000400294
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Hepatitis A: the costs and benefits of the disease prevention by vaccine, Paraná, Brazil BJID
Zahdi,Mariana Ribas; Maluf Junior,Ivan; Maluf,Eliane Mara Cesário Pereira.
This study evaluated the epidemiological behavior of the hepatitis A in Paraná state and compared the costs of the disease and the vaccination. This is an epidemiological descriptive study including a pharmacoeconomy analysis. We collected information in the national database reported cases (SINAN), in the mortality information system (SIM) and in the hospital information system (AIH) among 2000/2003 (Paraná State Public Health Department). We estimated the probability of one cohort of children to acquire hepatitis A during their lifetime and the costs with their treatment. We compared those costs with the cost of vaccinating the children. 14,682 hepatitis A cases were registered during the period studied, and 12,102 (82.4%) occurred in the 0-15 years-old...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hepatitis A; Vaccine; Epidemiology; Cost-benefit.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000400003
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Hepatitis C as a risk factor for diabetes type 2: lack of evidence in a hospital in central-west Brazil BJID
Costa,Luce Marina Freires Corrêa da; Mussi,Aparecida Duarte Hg; Brianeze,Marylina Rodrigues; Souto,Francisco José Dutra.
In order to assess the importance of HCV infection as a possible risk factor for type 2 diabetes mellitus, a case-control study was conducted, comparing the prevalence of HCV infection among diabetic and non-diabetic patients. Diabetic outpatients attending to a University Hospital in Central-West Brazil were evaluated between April and October 2005. A control group composed by patients from the same institution was matched by gender and age. Candidates to control group were included only if fasting glucose measures were under 100 mg/dL. Diabetics and controls had blood samples taken in order to test for antibodies against HCV (anti-HCV) by enzyme-immunoassay. Polymerase chain reaction and immunoblot were performed to confirm the anti-HCV status. Each...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Type 2 diabetes mellitus; Case control study; Risk factors; Hepatitis C virus; Epidemiology.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100007
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Hepatitis E virus seroprevalence among schistosomiasis patients in Northeastern Brazil BJID
Passos-Castilho,Ana Maria; de Sena,Anne; Domingues,Ana Lucia Coutinho; Lopes-Neto,Edmundo Pessoa; Medeiros,Tibério Batista; Granato,Celso Francisco Hernandez; Ferraz,Maria Lúcia.
Abstract Background Hepatitis E virus (HEV) can cause chronic infection with rapid progression to liver cirrhosis in immunocompromised patients. HEV seroprevalence in patients with Schistosoma mansoni in Brazil is unknown. We evaluated the prevalence of past or present HEV infection in schistosomiasis patients in Recife, Pernambuco, Brazil. A total of 80 patients with Schistosoma mansoni were consecutively enrolled in a cross-sectional study. Serum samples were tested for the presence of anti-HEV IgG antibodies by enzyme immunoassay (Wantai anti-HEV IgG, Beijing, China) and for the presence of HEV RNA using real time reverse transcriptase-polymerase chain reaction with primers targeting the HEV ORF2 and ORF3. Clinical and laboratory tests as well as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazil; Epidemiology; Hepatitis E virus; Schistosomiasis; Seroprevalence.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000300007
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High prevalence of hepatitis C associated with familial history of hepatitis in a small town of south Brazil: efficiency of the rapid test for epidemiological survey BJID
Ivantes,Cláudia Alexandra Pontes; Silva,Danilo; Messias-Reason,Iara.
This report describes a cross-sectional survey on the prevalence of hepatitis C antibodies (anti-HCV) in Tamboara, a small community in the northwest area from Paraná State, south of Brazil with a high rate of accumulated detection for HCV. Eight hundred and sixteen residents (17.87% from all the population), independently of the age and time living in Tamboara were included in this study by an epidemiologic questionnaire and by testing for anti-HCV. The rapid immuno-chromatographic test was applied for detection of HCV antibodies. The anti-HCV prevalence by rapid test was 4.28%. The median age for positive and negative test was 60.49 ± 14.14 and 41.67 ± 20.25, respectively (p < 0.001). By multivariate analysis, only familial history of hepatitis (p =...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hepatitis C; Epidemiology; Brazil.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000500010
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High sensitivity C-reactive protein distribution in the elderly: the Bambuí Cohort Study, Brazil BJMBR
Assunção,L.G.S.; Eloi-Santos,S.M.; Peixoto,S.V..
The measurement of the serum concentration of the acute-phase reactant C-reactive protein (CRP) provides a useful marker in clinical practice. However, the distribution of CRP is not available for all age and population groups. This study assessed the distribution of high sensitivity-CRP (hs-CRP) by gender and age in 1470 elderly individuals from a Brazilian community that participates in the Bambuí Cohort Study. Blood samples were collected after 12 h of fasting and serum samples were stored at -70°C. Measurements were made with a commercial hs-CRP immunonephelometric instrument. More than 50% of the results were above 3.0 mg/L for both genders. Mean hs-CRP was higher in women (3.62 ± 2.58 mg/L) than in men (3.03 ± 2.50 mg/L). This difference was observed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: C-reactive protein; Elderly; Epidemiology; Cardiovascular risk; Brazil.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200025
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Histopathological and molecular characterization of encephalitic listeriosis in small ruminants from northern Paraná, Brazil BJM
Headley,Selwyn Arlington; Bodnar,Lívia; Fritzen,Juliana T.T.; Bronkhorst,Dalton Evert; Alfieri,Alice Fernandes; Okano,Werner; Alfieri,Amauri Alcindo.
Listeriosis is a disease primarily of ruminants caused by the Gram-positive bacterium Listeria monocytogenes. Ruminants either demonstrate manifestations of the encephalitic, septicemic, or reproductive form of listeriosis. The pathological and molecular findings with encephalitic listeriosis in a 5.5-month-old, male, mixed-breed goat and a 3-year-old Texel-crossed sheep from northern Paraná, Brazil are described. Clinically, the kid demonstrated circling, lateral protrusion of the tongue, head tilt, and convulsions; the ewe presented ataxia, motor incoordination, and lateral decumbency. Brainstem dysfunctions were diagnosed clinically and listeriosis was suspected. Necropsy performed on both animals did not reveal remarkable gross lesions; significant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brainstem dysfunctions; Rhombencephalitis; Neuropathology; Listeriolysin gene; Epidemiology; Listeria monocytogenes.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300036
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HIV-1 mother-to-child transmission in Brazil (1994-2016): a time series modeling BJID
Coelho,Antonio Victor Campos; Coelho,Hemílio Fernandes Campos; Arraes,Luiz Cláudio; Crovella,Sergio.
Abstract HIV-1 mother-to-child transmission (HIV-1 MTCT), is an important cause of children mortality worldwide. Brazil has been traditionally praised by its HIV/Aids program, which provides free-of-charge care for people living with HIV-1. Using public epidemiology and demographic databases, we aimed at modeling HIV-1 MTCT prevalence in Brazil through the years (1994-2016) and elaborate a statistical model for forecasting, contributing to HIV-1 epidemiologic surveillance and healthcare decision-making. We downloaded sets of live births and mothers' data alongside HIV-1 cases notification in children one year old or less. Through time series modeling, we estimated prevalence along the years in Brazil, and observed a remarkable decrease of HIV-1 MTCT...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HIV-1; Vertical transmission; Perinatal transmission; Biostatistics; Epidemiology; Forecasting.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000400218
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Honeybees and caterpillars: epidemiology of accidents involving these animals in the Criciúma region, southern Santa Catarina State, Brazil J. Venom. Anim. Toxins incl. Trop. Dis.
Cristiano,MP; Cardoso,DC.
The present study aimed to acquire knowledge regarding some aspects of the epidemiology and injuries provoked by honeybees and caterpillars in southern Santa Catarina State, Brazil. Epidemiological information concerning accidents with both animals was prospectively collected from the System of Injury Notification Information (SINAN) files of the Municipal Health Secretary of the Criciúma region, Brazil. Between 1994 and 2006, 203 cases of accidents were registered, 149 provoked by honeybees (73.39%) and 54 by caterpillars (26.61%). The majority of the victims (200 cases) presented full recovery, two cases presented sequelae after recovery, and only one death was recorded in a case involving honeybee injury. The high frequency of victim recovery suggests...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epidemiology; Honeybees; Caterpillars; Poisonous animals; Accidents; Brazil.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400013
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Host colonization differences between citrus and coffee isolates of Xylella fastidiosa in reciprocal inoculation Scientia Agricola
Prado,Simone de Souza; Lopes,João Roberto Spotti; Demétrio,Clarice Garcia Borges; Borgatto,Adriano Ferreti; Almeida,Rodrigo Piacentini Paes de.
Citrus variegated chlorosis (CVC) and coffee stem atrophy (CSA) are important diseases in Brazil associated with closely-related strains of Xylella fastidiosa, but little is know about host aoverlappingnd importance of citrus and coffee as inoculum sources of these strains. In this study, reciprocal-inoculation experiments were performed to determine if CVC and CSA isolates are biologically similar within citrus and coffee plants. These two hosts were mechanically inoculated with a CVC and a CSA isolate of X. fastidiosa at four concentrations ranging between10³ and 10(9) colony forming units CFU mL-1. At two, four and eight months after inoculation, the infection efficiency and bacterial populations of the isolates in each host were determined by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus variegated chlorosis; Coffee leaf scorch; Inoculum concentration; Bacterial population; Epidemiology.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000300005
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How are HCV-infected patients being identified in Brazil: a multicenter study BJID
Portari-Filho,Luiz H.; Álvares-da-Silva,Mario R.; Gonzalez,Aline; Ferreira,Adalgisa P.; Nogueira,Cristiane V.; Mendes-Correa,Maria C.; Lima,José M.; Lopes,Edmundo P.; Brandão,Carlos E.; Ivantes,Cláudia; Lyra,André; Lindenberg,Andreia; Ferraz,Maria L..
ABSTRACT Background: Hepatitis C is an important health problem. In Brazil, 1-2 million people are infected. Despite this expressive number, and the availability of very successful treatment, many patients remained undiagnosed mainly because of the asymptomatic nature of the infection. Objectives: To describe epidemiological characteristics of HCV-infected patients seen at referral centers in Brazil, the source of referral, and the time spanned to reach a reference center, in order to improve the identification of undiagnosed patients. Methods: Multicenter observational, cross-sectional study carried out in 15 centers of Brazil, between January/2016 and June/2017. Data of patients with a confirmed diagnosis (anti-HCV and HCV-RNA) were collected by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epidemiology; Hepatitis C; HCV; Brazil.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000100034
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